[BioC] justMAS reliability
Crispin Miller
CMiller at PICR.man.ac.uk
Tue Jun 28 11:27:32 CEST 2005
I think I replied to this already?
Hi,
I've just had a look at this... I think you probably want a comma after
"1007_s_at"; exprs is a 2D matrix:
Try:
exprs(j)[gn=="1007_s_at",]
^
|
Note the comma
(exprs(j) should give you the same things as j at exprs)
I get the same results when I call justMAS more than once (trying to
duplicate your example).
If you're still having problems let me know - but with more details
(what version of R, what version of simpleaffy, what machine you're
running it on etc...). Hope that helps,
Cheers,
Crispin
-----Original Message-----
From: bioconductor-bounces at stat.math.ethz.ch
[mailto:bioconductor-bounces at stat.math.ethz.ch] On Behalf Of Eitan
Halper-Stromberg
Sent: 24 June 2005 20:51
To: bioconductor at stat.math.ethz.ch
Subject: [BioC] justMAS reliability
Hi,
I am trying to speed up my mas5 processing by using justMAS on my
affybatch. My AffyBatch is made of 8 chips and the cdf for these chips
is hgu133a. I have tested justMAS by running it several times on this
affybatch and I am obtaining different expression measure results even
though I am doing the exact same command. My affybatch is called 'F'
and an array of geneNames is called 'gn', and I type:
> j<-justMAS(F)
> j at exprs[gn=="1007_s_at"]
1007_s_at <NA> <NA> <NA> <NA> <NA>
<NA> <NA>
6.603113 6.171309 6.399581 6.171309 6.171309 6.171309 6.171309
6.036457
> i<-justMAS(F)
> i at exprs[gn=="1007_s_at"]
1007_s_at <NA> <NA> <NA> <NA> <NA>
<NA> <NA>
6.603113 NaN 6.171309 5.482231 6.171309 5.438713 5.387300
7.311159
Does anybody know why I am getting this discrepancy?
-Eitan
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