[BioC] GC-RMA - interpretation of the expression levels
Sean Davis
sdavis2 at mail.nih.gov
Fri Jun 10 14:38:23 CEST 2005
On Jun 10, 2005, at 8:26 AM, Pintilie, Melania wrote:
>
> For example I know that the expression levels (not logged) obtained
> using
> cDNA microarray can be interpreted (ideally)as: 1=no expression, >2
> over
> expression and <0.5 under expression.
>
Your best bet is to use statistical methods to determine expression in
1 group of experiments relative to another. Look at the vignette for
multtest and the user guide for Limma
(http://bioinf.wehi.edu.au/limma/usersguide.pdf) for examples.
Sean
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