[BioC] how to get the normalized genes expression values with vsn
Joern Toedling
toedling at ebi.ac.uk
Wed Jun 1 10:18:22 CEST 2005
diego huck wrote:
>
>
> Hello
>
> I normalized a data.frame with genes in rows and conditions in columns
> with vsn
>
> genormalized <- vsn(ge)
>
> latter I tried to get the normalized values with the following command
> but I didn't get the result I need
>
> genormalized$exprs
>
> NULL
Hi,
you use the dollar sign to get list components, but the expression
matrix is a slot of the exprSet and thus can be accessed by
'genormalized at exprs' or 'exprs(genormalized)' (have a look at
help(exprSet) to learn about other methods). The result can be written
by 'write.table', but most Bioconductor functions are able to handle
'exprSet' objects.
Regards,
Joern
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