[R] heatmap2 error key

[Catalina Aguilar Hurtado]

Hi I am trying to understand what happen with the heatmap.2 code that it
used to work (last used in October 2015). I am able to get a heatmap but my
colour key doesn't come up and instead I get an error in all my files the
used to work. Does anyone has any idea of what could have changed? or how
to fix this.

Thanks.

#

Error in seq.default(min.raw, max.raw, by = min(diff(breaks)/100)) :
  wrong sign in 'by' argument
In addition: Warning message:
In image.default(1:nc, 1:nr, x, xlim = 0.5 + c(0, nc), ylim = 0.5 +  :
  unsorted 'breaks' will be sorted before use

##

library("RColorBrewer")
library("gplots")

library ("gtools")

GO_Fil=read.table("1h-6h-up-down-v4.csv", sep=",", header=T)

data =data.matrix (GO_Fil[,3:4]) # define which columns to use for the
heatmap

rownames(data) <- GO_Fil$Description

summary (data)

pairs.breaks <- c(seq(-6, -0.01, length.out=50), seq(-0.02,
0.01,length.out=150), seq(0.02, 3.9,length.out=80))

hmcols<- colorRampPalette(c("blue","white", "red"))(length(pairs.breaks)-1)

pdf("Heat_1hv6h-updown-key.pdf",
    width = 15,
    height = 20,
    pointsize = 5)

hm<- heatmap.2(data, breaks=pairs.breaks, col=hmcols, na.color="white",
               trace="none",Colv=FALSE,
               dendrogram = "none",
               density.info="none",
               key="TRUE",
               keysize = 2,
               key.xlab = "Log2FC",
               key.title = "key",
               cexCol=3.0,
               cexRow=2,
               lwid= c(0.4,1),
               lhei = c(0.1, 4),
               margins= c(50,80),
               symm=F,symkey=F,symbreaks=T, scale="none")

dev.off()

> R.version
               _
platform       x86_64-apple-darwin13.4.0
arch           x86_64
os             darwin13.4.0
system         x86_64, darwin13.4.0
status
major          3
minor          2.2
year           2015
month          08
day            14
svn rev        69053
language       R
version.string R version 3.2.2 (2015-08-14)

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