[BioC] Quick question about Chromosome names using Tophat2 and Cufflinks w/Ensembl NCBIM37
Sindre Lee
sindre.lee at studmed.uio.no
Mon Mar 10 08:36:42 CET 2014
Tophat2 cmd:
tophat2 -o path/to
--transcriptome-index=/Mus_musculus_Ensembl_NCBIM37/Mus_musculus/Ensembl/NCBIM37/Annotation/Genes/genes
/Mus_musculus_Ensembl_NCBIM37/Mus_musculus/Ensembl/NCBIM37/Sequence/Bowtie2Index/genome
001.fastq.gz
Cufflinks cmd:
cufflinks --output-dir path/to --GTF-guide
/Mus_musculus_Ensembl_NCBIM37/Mus_musculus/Ensembl/NCBIM37/Annotation/Archives/archive-2013-03-06-18-55-12/Genes/genes.gtf
--frag-bias-correct
/Mus_musculus_Ensembl_NCBIM37/Mus_musculus/Ensembl/NCBIM37/Sequence/Bowtie2Index/genome.fa
--multi-read-correct --upper-quartile-norm --verbose accepted_hits.bam
Cufflinks then names the chromosomes like this, is that correct? How
can I control this or alter it?
20: MT
10: 18
11: 19
4: 12
5: 13
2: 10
3: 11
8: 16
9: 17
6: 14
7: 15
22: Y
21: X
19: 9
18: 8
17: 7
16: 6
15: 5
14: 4
13: 3
12: 2
1: 1
Thank you!
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