[BioC] [Help for DESeq]: Error in lfproc - newsplit: out of vertex space

[Imène SENINET]

Hello,

Thank you for having invovled me in this mailing list :)

I am trying to measure gene expression differences using RNAseq data. I 
did several runs using the package R/DESeq. For my testing data I used a 
*.cvs file, i.e. a table of raw counts containing more than 10800000 
reads with 2 conditions (no replicate)

I get the following error message after calling the function "cds 
<-estimateDispersions(cds, method='blind',sharingMode='fit-only', 
fitType='local')":

-------------------------------------------------------------------------------------------------------------------------------------- 

/Error in lfproc(x, y, weights = weights, cens = cens, base = base, geth
= geth,  :
newsplit: out of vertex space
In addition: There were 50 or more warnings (use warnings() to see the
first 50) /
--------------------------------------------------------------------------------------------------------------------- 


I've read that this error is related to maxk parameter value in locfit, 
so I increased it from 300 to 11000000. But the provided solutions did
not work.

Would you please help me to find out what wrong with my test?

Thank you very much and kind regards,

Imène


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