Hi Jose
not sure what is going on.
The best might be to take one of these reads (find the read ID in IGV,
then grep for it in the SAM file) and first check whether it might be
multiply aligned ("NH" field). Then, make a mini SAM file with only this
read and feed it to hseq-count, with the "--samout" option, to see what
the script does with it.
Simon