[BioC] readQpcrBatch function in qpcrNorm
Mueller, Alan
A.J.Mueller at liverpool.ac.uk
Tue Sep 4 16:25:55 CEST 2012
Dear List,
I am having difficulty reading in multiple text files using the 'readQpcrBatch' function from qpcrNorm. Reading in single files is not a problem:
> data=readQpcr("file1.txt",header=FALSE)
> slotNames(data)
[1] "geneNames" "plateIndex" "exprs" "normalized" "normGenes"
# However, trying to read all text files from an autonomous folder results in this error:
> data=readQpcrBatch()
Error in initialize(value, ...) :
no slot of name "primerNames" for this object of class "qpcrBatch"
#The columns conform to the requirements of readQpcr and I get this error even if I read in the data file 'qpcrBatch.object ' that is provided with the vignette as a text file.
#The same outcome results from:
> data=readQpcrBatch(filenames=c("file1.txt","file2.txt"),header=FALSE)
Error in initialize(value, ...) :
no slot of name "primerNames" for this object of class "qpcrBatch"
> sessionInfo()
R version 2.15.1 (2012-06-22)
Platform: x86_64-pc-mingw32/x64 (64-bit)
locale:
[1] LC_COLLATE=English_United Kingdom.1252
[2] LC_CTYPE=English_United Kingdom.1252
[3] LC_MONETARY=English_United Kingdom.1252
[4] LC_NUMERIC=C
[5] LC_TIME=English_United Kingdom.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] qpcrNorm_1.14.0 affy_1.34.0 limma_3.12.1 Biobase_2.16.0
[5] BiocGenerics_0.2.0
loaded via a namespace (and not attached):
[1] affyio_1.24.0 BiocInstaller_1.4.7 preprocessCore_1.18.0
[4] tools_2.15.1 zlibbioc_1.2.0
Any suggestions are gratefully received.
Regards
Alan
Alan James Mueller BSc BVM&S MRes MRCVS
Room 1.02
Comparative Musculoskeletal Research Group
The School of Veterinary Science
University of Liverpool
Leahurst
Chester High Road
Neston
CH64 7TE
UK
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