[BioC] LIMMA -- obtaining values used to calculate lfc
Sam McInturf [guest]
guest at bioconductor.org
Fri Jul 27 17:51:35 CEST 2012
Hello everyone,
I am working on a set of affymetrix arrays, and I am interested in obtaining the A and B values in lfc = log(A/B).
I wrote several small functions to annotate my topTable outputs, and one of these annotations was the exprs() for each probe, but when comparing the lfc's that topTable output, they are very different than the values that I could calculate with the exprs() values. So I am then lead to believe that the lfc is calculated by expression values that have been adjusted/better estimated by eBayes() and so on.
So can I take a lfc, A, M, exprs(), et cetera to get the values that the log fold change is directly calculated from?
Sorry if this question has been asked, I have no idea how to describe my problem succinctly.
Cheers!
Sam McInturf
-- output of sessionInfo():
> sessionInfo()
R version 2.15.0 (2012-03-30)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=English_United States.1252
[2] LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C
[5] LC_TIME=English_United States.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
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