[BioC] Reading Paired End Native Report Format in ShortRead
Murli Nair [guest]
guest at bioconductor.org
Thu Jul 12 20:42:32 CEST 2012
Hi,
I am trying to read the alignments generated using NovoAlign. The format I have the data is Paired End Native Report Format(http://computing.bio.cam.ac.uk/local/doc/NovoCraftV2.06.pdf).
What is the most efficient way to read this data into ShortRead? Since it is paired end data I have two files corresponding to the two sides.
I tried without success using the different formats using readAligned(). I also read an earlier posting about it which suggests to convert it to SAM format.
I would appreciate your suggestions.
Cheers../Murli
-- output of sessionInfo():
R version 2.15.0 (2012-03-30)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=C LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] ShortRead_1.14.4 latticeExtra_0.6-19 RColorBrewer_1.0-5
[4] Rsamtools_1.8.5 lattice_0.20-6 Biostrings_2.24.1
[7] GenomicRanges_1.8.7 IRanges_1.14.4 BiocGenerics_0.2.0
loaded via a namespace (and not attached):
[1] Biobase_2.16.0 bitops_1.0-4.1 grid_2.15.0 hwriter_1.3 stats4_2.15.0
[6] tools_2.15.0 zlibbioc_1.2.0
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