[BioC] Differential Gene expression analysis help

[James W. MacDonald]

Hi Srikanth,

Take a look at the limma User's Guide. There are multiple worked examples.

Best,

Jim



On 9/14/2011 6:02 AM, Srinivas M. Srikanth wrote:
> Hi Sean, Members,
>
> I am analyzing the microarray data from exposed vs unexposed to metals. The
> data is single color Agilent platform. I normalized all the arrays and
> exported as a text file all the green intensities. Now I need to find which
> are the differentially expressed genes from this single file. How to proceed
> please help?
>
> File Headers for normalized data:
> ProbeName Genesymbol GeneName Exposed1 Exposed2......Unexposed1
> Unexposed2...
>
> It would be helpful if you let me know the exact commands as I am a newbie.
>
> Regards,
> Srikanth
>
>
>
>

-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826

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