[BioC] EBarrays question
jgomez at uni-potsdam.de
jgomez at uni-potsdam.de
Fri Apr 8 11:08:03 CEST 2011
Dear List,
I would like to use EBarrays to analyse 24 MAs without replicates. I
have 12 samples taken at one time point, and 12 taken at other time
point. I want to find the genes differentially express at one time
point vs. the other.
I have chosen EBarrays because I don't have replicates. Until the
fitting of the model (emfit) it seems to work. However, when I want to
check the post. probabilities I get NULL. I have tried all kinds of
things I could think of. The answer is always:
gg_post_IA_P01.out
NULL
if I understand right, most genes belong to the µ1=µ2 pattern
Pattern.1 Pattern.2
Cluster 1 0.9838123 0.01618766
But no matter how I set the pattern (including all MAs, comparisons of
only one time point at a time, etc), the answer is always NULL for
post. prob.
Does anyone has any idea how to set my analysis, or at the very least
why the calculation of post. probs always renders NULL?.
Thanks in advance.
Regards,
Judith
--
Judith Lucia Gomez, PhD
Universität Potsdam
BioPhysics and Molecular Plant Biology
Heisenberg-Group
D-14476 Potsdam
Germany
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