[BioC] Ecoli antisense library

James W. MacDonald jmacdon at med.umich.edu
Wed Mar 17 17:06:11 CET 2010


Hi Bala,

Try this:

eset <- justRMA(cdfname = "ecoliasv2cdf")

Best,

Jim



Balasubramanian Ganesan wrote:
> Hello BioC team
> I have downloaded an old microarray expression data set for analysis from
> NCBI's GEO database.
> When I try to normalize the data in R2.9 (OS X Leopard) using justRMA() the
> normalization fails as it is looking for a library file called
> "ecoliantisensecdf"
> and cannot find it.
> However if try to download the corresponding library file from Affy''s
> website I only get a library file called "Ecoli_ASv2" and not
> "ecoliantisense".
> Affymetrix insists that this library file works fine with the cel files I
> have inside their software (Expression console) and that the array layouts
> of the older ecoliantisense and the newer Ecoli_ASv2 are the same. But I
> have had no luck trying to use the new library in R.
> Is there a possible way to resolve this issue?
> Thank you inn advance.
> BALA
> 
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