[BioC] Affymetrix miRNA array normalization
Kamila Naxerova
naxerova at fas.harvard.edu
Sat Jul 10 17:26:43 CEST 2010
Dear list,
I was searching the archives for discussions about the Affymetrix miRNA
chip... there are only a few threads and most of them are almost a year
old. I was wondering whether in the meantime people had gathered some
experience with analysis strategies - normalization in particular - and
were willing to share some insights. I do not feel comfortable using RMA
because I doubt that total miRNA expression can be assumed to be
constant across samples (particularly when studying developmental
processes or cancer). Has anybody played with the options?
Thanks!
Kamila
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