[BioC] HTqPCR - how to read data file with multiple samples

Marco Fabbri fabbri.marco at gmail.com
Tue Feb 16 09:46:55 CET 2010


Hi,
   I am trying ti import into HTqPCR a TLDA with two sample, I have
seen a previous post but I ma not able to make it works, it
recogniseas a single sample but I have a two samples.

I have isntalled HTqPCR 1.1.o on a windows machine


#  	Plate	Pos	Flag	Sample	Detector	Task	Ct
1	Control	A1	Passed	Sample01	Gene1	Endogenous Control	11.463166
2	Control	A2	Passed	Sample01	Gene2	Target	33.949196
3	Control	A3	Passed	Sample01	Gene3	Target	27.956657
4	Control	A4	Passed	Sample02	Gene1	Target	26.92745
5	Control	A5	Passed	Sample02	Gene2	Target	24.992647
6	Control	A6	Flagged	Sample02	Gene3	Target	11.280299

An object of class "qPCRset"
Size:  384 features, 1 samples
Feature types:           Endogenous Control, Target
Feature names:           CYP1A1-Hs00153120_m1 CYP1A1-Hs00153120_m1
CYP1A1-Hs00153120_m1 ...
Feature classes:
Feature categories:      OK, Undetermined
Sample names:            Documents and
Settings/fabbrmc/Desktop/card1/card1 NA NA ...


Thank you

Marco



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