[BioC] Nonspecific filtering on Human Gene ST 1.0 Arrays
Javier Pérez Florido
jpflorido at gmail.com
Tue Nov 24 17:46:51 CET 2009
Dear list,
I've tried to use the nsFilter function from genefilter package to
perform nonspecific filtering on a Human Gene ST 1.0 dataset.
The expression set has the hugene10sttranscriptcluster.db annotation
package, but when nsFilter is applied:
nsFilter(subEset,var.cutoff=0.5) # Removes AFFX genes, those without
EntrezGene IDs, etc
Error is do.call(paste(chip, "_dbconn", sep = ""), list()) :
"hugene10sttranscriptcluster.db_dbconn" cannot be found
Maybe nsFilter is not built for this kind of microarrays, although
varFilter,from the same package, works, because it makes use of the
expression values only.
I've made some search on Google, but no success. I can write a function
that removes the different control genes in Human Gene (AFFX, spike,
neg_control,pos_control,etc) and those without EntrezGene ID, but wanted
to know if there is a function that handle this for this kind of
microarrays.
Thanks,
Javier
sessionInfo()
R version 2.10.0 (2009-10-26)
i386-pc-mingw32
locale:
[1] LC_COLLATE=Spanish_Spain.1252 LC_CTYPE=Spanish_Spain.1252
[3] LC_MONETARY=Spanish_Spain.1252 LC_NUMERIC=C
[5] LC_TIME=Spanish_Spain.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] Matrix_0.999375-31
lattice_0.17-26
[3] ALL_1.4.7
genefilter_1.28.0
[5] pd.hugene.1.0.st.v1_3.0.0
Category_2.12.0
[7] KEGG.db_2.3.5
hugene10sttranscriptcluster.db_4.0.1
[9] org.Hs.eg.db_2.3.6
RSQLite_0.7-3
[11] DBI_0.2-4
oligo_1.10.0
[13] preprocessCore_1.8.0
oligoClasses_1.8.0
[15] GSEABase_1.8.0
graph_1.24.0
[17] annotate_1.24.0
AnnotationDbi_1.8.0
[19] Biobase_2.6.0
loaded via a namespace (and not attached):
[1] affxparser_1.18.0 affyio_1.14.0 Biostrings_2.14.0 grid_2.10.0
[5] IRanges_1.4.0 RBGL_1.20.0 splines_2.10.0 survival_2.35-7
[9] tools_2.10.0 XML_2.6-0 xtable_1.5-5
>
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