[BioC] flowViz xyplot smooth=F gate outline ONLY
Aric Gregson
a.gregson at ucla.edu
Thu Jun 25 01:15:57 CEST 2009
Hello,
I am trying to use code from a presentation of Deepayan that I found
on the web. The code does a nice job a presenting density information in
the flowViz xyplot utilizing a more traditional appearance as found in
other flow software while still ploting all points. I would like to
use this as an occasional alternative to relying upon the alpha setting
to depict density. With this code, I would like to:
1) ONLY outline the gated cells rather than color and outline the
cells. I have not found a way to do this. The best I have managed is in
the example below, which overdraws with a light rose color. Setting
fill = "transparent" appears to have no effect.
2) Apply this type of plot to a flowSet. I have not figured this out
yet either. Replacing panel.xyplot.flowframe with panel.xyplot.flowset
fails.
Any hints would be greatly appreciated.
xyplot(`Indo.1..Violet..A` ~ `APC.H7.A`, Data(wf[["logicle"]])
[[4]],
smooth=F,
pch=21,
cex=0.3,
aspect="iso",
panel = function(x, y, col, ...) {
cols <-
densCols(x, y,
nbin=128,
colramp = colori)
panel.xyplot(x, y, col = cols, ...)
panel.xyplot.flowframe(x, y, filter=livefilter, outline=TRUE,
names=TRUE, col="transparent", ...) },
par.settings=list(gate=list(col= cols, fill= cols, alpha=.9),
gate.text=list(col="black", alpha=0.7, cex=0.6)))
Thanks in advance.
Aric
============================
R version 2.8.0 (2008-10-20)
i386-pc-solaris2.11
locale:
C
attached base packages:
[1] tools stats graphics grDevices utils datasets
methods [8] base
other attached packages:
[1] geneplotter_1.20.0 annotate_1.20.1 xtable_1.5-4
[4] AnnotationDbi_1.4.1 colorspace_1.0-0 flowViz_1.7.10
[7] lattice_0.17-20 flowCore_1.9.18 rrcov_0.4-07
[10] robustbase_0.4-3 Biobase_2.2.1 Hmisc_3.4-4
loaded via a namespace (and not attached):
[1] DBI_0.2-4 KernSmooth_2.22-22 MASS_7.2-44
RColorBrewer_1.0-2 [5] RSQLite_0.7-1 cluster_1.11.11
feature_1.1-14 graph_1.20.0 [9] grid_2.8.0
latticeExtra_0.5-4 stats4_2.8.0 tcltk_2.8.0
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