[BioC] Variance
David martin
vilanew at gmail.com
Mon Jun 22 14:55:48 CEST 2009
Hello,
I'm newbie with R.
I have a dataframe as follows. The microarray data has been normalized
using spike in controls and the values shown are normalized log2 values.
CondA CondB CondC CondD CondE
geneA -6.19 -5.74 -5.82 -5 -5.59
geneB -6.33 -5.32 -5.6 -4.88 -5.39
geneC -6.15 -6.07 -5.6 -4.88 -5.9
geneD -6.57 -6.11 -6.36 -5.36 -5.96
geneD -6.74 -6.2 -5.49 -5.35 -5.95
geneE -6.75 -6.24 -5.73 -5.63 -6.02
From this small subset (in fact i have a larger dataframe) i would like
to know which gene has the lowest variance. I would like to see is some
of these genes could be used as reference genes for RT-PCr experiments.
How can i plot and get the variance from this genes and see what would
the best candidates to be used as reference genes.
thanks,
david
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