[BioC] PLIER -- low values?

Tarun Nayar tnayar at bcgsc.ca
Fri May 27 23:29:04 CEST 2005


Hi,
 
I've been trying to use PLIER to pre-process microarray data from 5 Rat
230A chips.
 
After using the justPlier function, I get an exprSet, but all of the
probe set intensities are below 12. When I've used PLIER previously with
the same data set(with Affy's free ArrayAssist Lite program), values are
much higher. I'm assuming there is some kind of a log function happening
in the R version. Is there any way to turn the log function off? I've
looked through the options but can't find anything that seems to make
this happen. We're presently using R to compare some data with a
collaborator who is using ArrayAssist.
 
Thanks,
 
Tarun Nayar
Genome Sciences Centre
Vancouver, BC

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