[BioC] normalization in limma

[cxd55 at cwru.edu]

Dear all:

I'm trying to use limma package. But my intensity data is not two-colored, but single color(gray), like the following:

Gene1   2596.87    2429.06    2436.62  2716.21      2508.85     2391.20          2515.86     2583.28
Gene2   1083.47    1071.37    1087.75  1022.47      1144.77     1078.31          1007.18     841.17
         

How should I do the normalization in Limma? Any guidance is appreciated.

Chunrong